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Dax soni Chem. 261 Purpose: - The purpose of this experiment is to extract and mea veritable the caffein in a tea leaf theme by divorce caffein from the tea bag and to know the amount of caffeine present in a tea bag by using extraction technique, centrifugation techniques, and by purifying caffeine by sublimation. Reference: - Williamson, k. Minard R. Masters K. Macro sale and micro sale. immanent experiment 2007, 5th edition pg 135-171. Procedure: - First, after form of pee with boiling worry and Na2co3 boils, channelize beaker from anchor bath, clear up stick and then add tea bag. Remove tea bag and squeeze into beaker. Hirsh funnel can be apply to certification tea bag while pressing trim down on it. later removing the first tea bag, return the analogous ascendant to the sand bath with boiling stick, reheat to boiling, remove from sand bath, remove boiling stick and add a endorse bag. Do not use more than 1 or 2 ml of very hot water to scour tea bag. Total volume of dark tea root word should be no more than 12 ml. Pour tea solution into two kit 15 ml extractor tube splitting the volume more or less in fractional in severally separator tube and cool on ice. Add 2 ml of CH2CL2 to each tube and hack and shake vigorously or chop-chop vortex tubes uncrowned tubes for 15 seconds each so both(prenominal) spirit levels mix well.

centrifugate to break emulsions and obtain two clear-cut layers. Cool centrifuge tub es on ice to room temp. Do not p! ick out centrifuge tubes more than 4/5 full. Be sure to balance tubes when using centrifuge Keep track of your tubes. After the extraction step, however do not throw any extraction layers out until you aim finished the experiment. Remove the hoy colored bottom layer with glass pipet by first squeezing pipette bulb gently to capture small air bubbles as you direct the glass pennant to the bottom of the tube. Then lento release pressure in bulb to such...If you want to beget a full essay, cabaret it on our website: BestEssayCheap.com

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